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Windows Logon error 1326 is defined as 'unknown user name or bad password'. This is different than the error 1385 that you get when you don't have the necessary rights. Error 1385 is defined as 'Requested logon type cannot be granted on this computer'.
If you are using IIS5 isolation mode, IIS should be respecting the machine.config settings rather than using the app pool security as far as I'm aware. Settings the process model security to MACHINE will cause the web app to run as ASPNET. If you do not manually set the password for ASPNET, the password in machine.config should be AUTOGENERATE, otherwise you should make sure the password here matches the one you have set.
If you set the ASPNET password using an encrypted BLOB in the registry, ANTS Profiler cannot log on as the ASPNET user and you'll need to at least temporarily place the password in the machine.config.
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